DETECTION METHODS TO TRACK ADENOVIRUS INACTIVA
Virion detection methods are important to ensure our wellbeing, investigate outbreaks, and devise preventive measures. We investigated the capability of three detection methods, cell culture plaque assay, nested PCR and enzyme-linked immunosorbent assay (ELISA), to assess the presence of infectious human adenovirus viral particles after inactivation by gamma radiation in different aqueous matrices. Among the tested methods, plaque assay was the only one that allowed tracking of the inactivation of the adenovirus strain by gamma radiation. This study is part of a wider research that aims to provide new insights on the inactivation of enteric viruses by gamma irradiation and to introduce new concepts on the utility of radiation technologies as effective tools to guarantee the reduction of viral pathogens.
Detection of Human Adenovirus Type 5 (i) in Tap water, Fetal Bovine Serum (FBS) and Wastewater after gamma radiation treatment (ii) (0.9 up 10.8 kGy) by three different methods: iii) Plaque assay; iv) Nested-PCR (A- 1st PCR round; B – 2nd PCR round) and v) ELISA using a monoclonal antibody to the hexon antigen of human adenovirus.
Pimenta, A.I.; Guerreiro, D.; Madureira, J.; Margaça, F.M.A.; Cabo Verde, S. (2016), Tracking human adenovirus inactivation by gamma radiation under different environmental conditions. Applied and Environmental Microbiology 82, 5166 –5173. DOI: 10.1128/AEM.01229-16.
Pereira, E.; Pimenta, A. I.; Calhelha, R. C.; Antonio, A. L.; Cabo Verde, S.; Barros, L.; Santos-Buelga, C.; Ferreira, I.C.F.R. (2016). Effects of gamma irradiation on cytotoxicity and phenolic compounds of Thymus vulgaris L. and Mentha x piperita L. LWT - Food Science and Technology 71: 370-377. DOI: 10.1016/j.lwt.2016.04.004