RADIOBIOLOGY AND BIOLOGICAL EFFECTS OF IONIZING RADIATION STUDIES
1) Based on the DNA-intercalation properties of Acridine Orange (AO), our research work aimed to study the ability of 125I-labelled AO-derivatives to cause DNA double-strand breaks (DSB) and the effect of the distance between the AO moiety and the radionuclide, in order to evaluate the utility of these AO-derivatives for Auger therapy.
Immunofluorescence image of γ-H2AX assay after incubation of PC-3 cells with 125I-C5 (left); Number of γ-H2AX foci per cell upon irradiation with 125I-C5 (right).
2) Quantification of DSB induction, using ɣ-H2AX assay, after incubation of U87 cells with compounds 1, 2 and 3 for BNCT studies.
Cellular uptake (up), obtained by fluorescence imaging, and number of Mn per BN cell (down) for compound 2 (A) and 3 (B).
Contact persons: Octávia Monteiro Gil, Ana Belchior